Apostle MiniMax® High Efficiency Cell-Free RNA Isolation Kit

  • Extracts cell-free RNA (cfRNA) ≥17 nt, including miRNA and other small RNAs, from plasma or serum samples in manual or automated mode
  • Uses Apostle MiniMax® magnetic nanoparticle technology (no phenol or chloroform needed)
  • Supports various blood collection tubes
  • Yields cfRNA suitable for a broad range of subsequent methods, including sequencing and PCR
  • Enables applications in liquid biopsy research, cancer research and biomarker discovery
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D30452, Apostle MiniMax® High Efficiency Cell-Free RNA Isolation Kit, 200 μL × 50 preps
D30451, Apostle MiniMax® High Efficiency Cell-Free RNA Isolation Kit, 1 mL × 50 preps

Data and Performance

Charts demonstrating recovery of RNA Ladders from serum and plasma with the Apostle Apostle MiniMax® High Efficiency Cell-Free RNA Isolation Kit

Figure 1. RNA ladder (100-1,000 nt) and small RNA ladder (17-150 nt) were spiked in serum or plasma respectively, followed by extraction with Apostle MiniMax® High Efficiency cfRNA Isolation Kit (red curve), and compared with original RNA ladder (blue curve) using Bioanalyzer 2100 RNA pico kit for 100-1,000 nt (A) and small RNA kit for 17-150 nt fragments (B). Apostle MiniMax® cfRNA Kit offers superior RNA and small RNA recovery efficiency of > 90%.

Charts demonstrating higher recovery of cfRNA with Apostle MiniMax® High Efficiency Cell-Free RNA Isolation Kit in comparison to a column-base competitor

Figure 2. cfRNA was isolated from plasma using Apostle MiniMax® High Efficiency cfRNA Isolation Kit (red) or a major column-based competitor (green). A synthetic RNA mimic cel-miR-39 was spiked in during the extraction procedure as the reference to evaluate recovery rate. The qPCR amplification plot and Ct value show 1.1, 9, and 2,000 times higher RNA recovery rate when compared to the column-based major alternative product for plasma collected in EDTA tube, cfRNA Blood Collection Tube (BCT) Vendor 1, or cfRNA BCT Vendor 2, respectively.

Charts demonstrating higher recovery of cfRNA extracted from samples collected to different blood collection tubes using Apostle MiniMax® cfRNA Kit in comparison to a column-base competitor and the spike-in control

Figure 3. cfRNA was isolated from plasma using Apostle MiniMax® High Efficiency cfRNA Isolation Kit (red) or a major column-based competitor (green). A synthetic RNA mimic cel-miR-39 was spiked in during the extraction procedure as the reference to evaluate recovery rate. The qPCR amplification plot and Ct value show higher RNA recovery rate when compared to the column-based major alternative product for plasma collected in three BCT types: EDTA, PAXgene, and Streck cfRNA BCTs, respectively.

Specifications

Application Extraction of cfRNA ≥17 nt
Technology Apostle MiniMax® magnetic nanoparticle technology
Recovery > 90%
Sample Amount 0.1–1 mL (scalable)
Sample types Plasma, serum
Processing mode Automated or manual
Kit Sizes
  • 200 μL x 50 preps
  • 1 mL x 50 preps
Kit Components and Storage Conditions Magnetic Nanoparticles (2 to 30°C), Proteinase K (2 to 30°C), Lysis/Binding Solution (15 to 30°C, in dark), Protein Precipitation Solution (15 to 30°C, in dark), Wash Solution (15 to 30°C, in dark), Elution Solution (15 to 30°C, in dark), Binding Enhancer (-25 to -15°C)

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D30453, Apostle MiniMax® High Efficiency Cell-Free DNA/RNA (cfNAs) Isolation Kit, 1 mL × 50 preps

cfDNA/cfRNA (cfNAs) Isolation Kit

Extracts cfDNA ≥80 bp and cfRNA ≥17 nt, including miRNA and other small RNAs, from plasma or serum samples.

Products and demonstrated applications are not intended or validated for use in diagnostic procedures.